Affiliation:
1. Laboratório de Micologia, Instituto de Pesquisa Clínica Evandro Chagas, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brazil
2. Molecular and Environmental Biology Centre (CBMA), Universidade do Minho, Braga, Portugal
Abstract
ABSTRACT
This article describes PCR fingerprinting using the universal primer T3B to distinguish among species of the
Sporothrix
complex,
S. brasiliensis
,
S. globosa
,
S. mexicana
, and
S. schenckii
. This methodology generated distinct banding patterns, allowing the correct identification of all 35 clinical isolates at the species level, confirmed by partial calmodulin (CAL) gene sequence analyses. This methodology is simple, reliable, rapid, and cheap, making it an ideal routine identification system for clinical mycology laboratories.
Publisher
American Society for Microbiology
Cited by
47 articles.
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