A Molecular-Beacon-Based Multiplex Real-Time PCR Assay To Distinguish Mycobacterium abscessus Subspecies and Determine Macrolide Susceptibility-Reference-Cited by-同舟云学术

A Molecular-Beacon-Based Multiplex Real-Time PCR Assay To Distinguish Mycobacterium abscessus Subspecies and Determine Macrolide Susceptibility

Published:2021-07-19 Issue:8 Volume:59 Page:
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Marras Salvatore A. E.¹,Chen Liang²³^ORCID,Shashkina Elena²,Davidson Rebecca M.⁴,Strong Michael⁴,Daley Charles L.⁵⁶,Kreiswirth Barry N.²^ORCID

Affiliation:

1. Public Health Research Institute Center, New Jersey Medical School, Rutgers University, Newark, New Jersey, USA

2. Center for Discovery and Innovation, Hackensack Meridian Health, Nutley, New Jersey, USA

3. Department of Medical Sciences, Hackensack Meridian School of Medicine, Nutley, New Jersey, USA

4. Center for Genes, Environment and Health, National Jewish Health, Denver, Colorado, USA

5. Department of Medicine, National Jewish Health, Denver, Colorado, USA

6. The University of Colorado, Aurora, Colorado, USA

Abstract

Mycobacterium abscessus is a rapidly growing nontuberculous mycobacterial species that comprises three subspecies: M. abscessus subsp. abscessus , M. abscessus subsp. massiliense , and M. abscessus subsp. bolletii . These predominantly environmental microorganisms have emerged as life-threatening chronic pulmonary pathogens in both immunocompetent and immunocompromised patients, and their acquisition of macrolide resistance due to the erm (41) gene and mutations in the 23S rrl gene has dramatically impacted patient outcome.

Funder

Cystic Fibrosis Foundation

Division of Intramural Research, National Institute of Allergy and Infectious Diseases

HHS | NIH | National Cancer Institute

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.00455-21

Reference45 articles.

1. Mycobacterium abscessusComplex Infections in Humans