Affiliation:
1. Department of Food Science and Nutrition, University of Minnesota, St. Paul, Minnesota 55101
Abstract
Ultraviolet (UV)-induced phage lysates, from lactose-positive (
lac
+
)
Streptococcus lactis
C2, transduced lactose fermenting ability to
lac
−
recipient cells of this organism. Although the phage titer could not be determined due to the absence of an appropriate indicator strain, the number of transductants was proportional to the amount of phage lysate added. Treatment of the lysate with deoxyribonuclease had no effect on this conversion, indicating the observed genetic change was not mediated by free deoxyribonucleic acid. When the
lac
+
transductants were isolated and exposed to UV irradiation, lysates with higher transducing ability were obtained. The transducing ability of this lysate was about 100-fold higher than that observed in the original lysates. The
lac
+
transductants were unstable since
lac
−
segregants occurred at high frequency. The phage lysate from
S. lactis
C2 also transduced maltose and mannose metabolism to the respective negative recipient cells. The results demonstrate the transduction of carbohydrate markers by a streptococcal phage and establish a genetic transfer system in group N streptococci.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Cited by
79 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献