Validation of Swab Sampling and SYBR Green-Based Real-Time PCR for the Diagnosis of Cutaneous Leishmaniasis in French Guiana-Reference-Cited by-同舟云学术

Validation of Swab Sampling and SYBR Green-Based Real-Time PCR for the Diagnosis of Cutaneous Leishmaniasis in French Guiana

Published:2021-01-21 Issue:2 Volume:59 Page:
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Blaizot Romain¹²³^ORCID,Simon Stéphane²³⁴,Ginouves Marine²,Prévot Ghislaine²,Blanchet Denis²⁴,Ravel Christophe⁵,Couppie Pierre¹²³,Demar Magalie²³⁴,Nabet Cécile⁴⁶

Affiliation:

1. Department of Dermatology, Andrée Rosemon Hospital, Cayenne, French Guiana

2. Tropical Biome and Immunophysiopathology (TBIP), Université de Lille, CNRS, INSERM, Institut Pasteur de Lille, U1019-UMR9017-CIIL-Centre d'Infection et d’Immunité de Lille, Centre Hospitalier de Cayenne, Université de Guyane, Cayenne, French Guiana

3. National Reference Centre for Cutaneous Leishmaniasis (associate laboratory), Cayenne, French Guiana

4. Laboratory of Parasitology-Mycology, Andrée Rosemon Hospital, Cayenne, French Guiana

5. University of Montpellier, National Reference Centre for Leishmaniasis, University Hospital Centre of Montpellier, MiVEGEC, Montpellier, France

6. Sorbonne Université, INSERM, Institut Pierre-Louis d’Epidémiologie et de Santé Publique, AP-HP, Groupe Hospitalier Pitié-Salpêtrière, Service de Parasitologie-Mycologie, Paris, France

Abstract

Recent studies have highlighted the interest in noninvasive sampling procedures coupled with real-time PCR methods for the detection of Leishmania species in South America. In French Guiana, the sampling method still relied on skin biopsies. Noninvasive protocols should be tested on a large annual cohort to improve routine laboratory diagnosis of cutaneous leishmaniasis. Therefore, we evaluated the performance of a new Leishmania detection and species identification protocol involving cotton swabs and SYBR green-based real-time PCR of the Hsp70 gene, coupled with Sanger sequencing.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.02218-20

Reference27 articles.

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