Exploiting a Natural Auxotrophy for Genetic Selection-Reference-Cited by-同舟云学术

Exploiting a Natural Auxotrophy for Genetic Selection

Published:2012-06-01 Issue:16 Volume:78 Page:5926-5928
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl. Environ. Microbiol.

Author:

Ramage Elizabeth,Gallagher Larry,Manoil Colin

Abstract

ABSTRACTWe exploited the natural histidine auxotrophy ofFrancisellaspecies to develophisD(encodes histidinol dehydrogenase) as a positive selection marker. A shuttle plasmid (pBR103) carryingEscherichia coli hisDand designed for cloning of PCR fragments replicated in both attenuated and highly virulentFrancisellastrains. During this work, we formulated a simplified defined growth medium forFrancisella novicida.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

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