Altered Metabolic Flux due to Deletion of odhA causes l -Glutamate Overproduction in Corynebacterium glutamicum

Author:

Asakura Yoko1,Kimura Eiichiro1,Usuda Yoshihiro1,Kawahara Yoshio1,Matsui Kazuhiko1,Osumi Tsuyoshi1,Nakamatsu Tsuyoshi2

Affiliation:

1. Ajinomoto Co., Inc., 1-1 Suzuki-cho, Kawasaki-ku, Kawasaki, Kanagawa 210-8681

2. Department of Environmental Material Science, Tokyo Denki University, 2-2 Kandanishiki-cho, Chiyoda-ku, Tokyo 101-8457, Japan

Abstract

ABSTRACT l -Glutamate overproduction in Corynebacterium glutamicum , a biotin auxotroph, is induced by biotin limitation or by treatment with certain fatty acid ester surfactants or with penicillin. We have analyzed the relationship between the inductions, 2-oxoglutarate dehydrogenase complex (ODHC) activity, and l -glutamate production. Here we show that a strain deleted for odhA and completely lacking ODHC activity produces l -glutamate as efficiently as the induced wild type (27.8 mmol/g [dry weight] of cells for the ohdA deletion strain compared with only 1.0 mmol/g [dry weight] of cells for the uninduced wild type). This level of production is achieved without any induction or alteration in the fatty acid composition of the cells, showing that l -glutamate overproduction can be caused by the change in metabolic flux alone. Interestingly, the l -glutamate productivity of the odhA -deleted strain is increased about 10% by each of the l -glutamate-producing inductions, showing that the change in metabolic flux resulting from the odhA deletion and the inductions have additive effects on l -glutamate overproduction. Tween 40 was indicated to induce drastic metabolic change leading to l -glutamate overproduction in the odhA -deleted strain. Furthermore, optimizing the metabolic flux from 2-oxoglutarate to l -glutamate by tuning glutamate dehydrogenase activity increased the l -glutamate production of the odhA -deleted strain.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Cited by 116 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3