Identification of the Cloned Gene for the Murine Transplantation Antigen H-2Kbby Hybridization with Synthetic Oligonucleotides

Abstract

The H-2Kbgene is a member of the large major histocompatibility complex class I gene family. Since many members of this family cross-hybridize with class I cDNA probes, the cloned H-2Kbgene was identified by hybridization with specific oligonucleotide probes. This clone was definitively shown to encode the H-2Kbpolypeptide by partial DNA sequencing and by serological and tryptic peptide analyses of the expressed product.