Separation and Identification of the Polar Lipids of Chromatium Strain D

Author:

Steiner S.1,Conti S. F.1,Lester R. L.1

Affiliation:

1. Department of Microbiology and Department of Biochemistry, University of Kentucky, Lexington, Kentucky 40506

Abstract

The polar lipids of the autotrophically grown, obligately anaerobic, photosynthetic bacterium Chromatium strain D were separated by paper chromatography. Four major phospholipids were identified: lysophosphatidylethanolamine, phosphatidylethanolamine, phosphatidylglycerol, and cardiolipin. In addition, three glycolipids were observed and characterized, namely, monoglucosyldiglyceride, which is found in other biological systems, and (mannosyl, glucosyl)-diglyceride and (dimannosyl, glucosyl)-diglyceride, which heretofore have not been observed in nature.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Reference15 articles.

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4. A simple, specific spray for the detection of phospholipids on thin layer chromatograms;Lester;J. Lipid Res.,1964

5. Fuller R. C. 1963. Media for anaerobic growth of photosynthetic bacteria p. 507-509. In H. Gest A. San Pietro and L. P. Vernon (ed.) Bacterial photosynthesis. The Antioch Press Yellow Spring Ohio.

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