Affiliation:
1. Department of Biological Sciences, University of Texas, El Paso, Texas 79968
2. Houston School of Public Health, University of Texas, Houston, Texas 77902
Abstract
ABSTRACT
For this study, we isolated
Helicobacter pylori
from wastewater by a series of steps beginning with immunomagnetic separation and cell culture. After Gram staining and three standard microbial tests, the 16S rRNA sequences of a total of 23 out of 37 putative
H. pylori
isolates were verified by PCR. Eleven
H. pylori
isolates were genotyped and fell into four
vacA
classes: those with the
vacA
allelic variants
s1a
and
m1
,
s1b
and
m1
,
s2
and
m2
, or
s2
and
m1
. Most
H. pylori
isolates were of the
vacA s1a
/
m1
type, which has been shown to be associated with advanced diseases based on genotyping of
H. pylori
from gastric cancer patients. These results demonstrated that
H. pylori
survives in water and may be a potential source of
H. pylori
transmission, especially where water is not adequately treated.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
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5. vacA
Genotypes in
Helicobacter pylori
Strains Isolated from Children with and without Duodenal Ulcer in Brazil
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