Pyrazinamidase activity in Yersinia enterocolitica and related organisms

Abstract

Pyrazinamidase activity was tested in 381 Yersinia strains from various ecological and geographical origins and belonging to the following species: Y. enterocolitica (five biogroups), Y. intermedia, Y. frederiksenii, Y. kristensenii, Y. aldovae, Y. pseudotuberculosis, and Y. pestis. The pyrazinamidase test was negative (Pyz-) in all bioserogroups of Y. enterocolitica, in which is usually harbored the virulence plasmid, and was involved in human or animal diseases. Y. pseudotuberculosis and Y. pestis were also Pyz-. The more ubiquitous bioserogroups of Y. enterocolitica, without naturally occurring virulence plasmid, and related species were all Pyz+. Pyrazinamidase activity allowed the separation of the pathogenic North American Y. enterocolitica isolates from other nonpathogenic strains within biogroup 1. Similarly, environmental biogroups 3A and 3B were clearly distinguished from pathogenic biogroup 3. However, the pyrazinamidase test was not linked to the presence of the virulence plasmid itself and should not replace the pathogenicity tests to assess the actual virulence of an individual strain. This test proved to be a valuable tool to distinguish potential pathogenic from nonpathogenic strains of Y. enterocolitica in epidemiological surveillance programs.