Case Study of the Distribution of Mucosa-Associated Bifidobacterium Species, Lactobacillus Species, andOther Lactic Acid Bacteria in the HumanColon-Reference-Cited by-同舟云学术

Case Study of the Distribution of Mucosa-Associated Bifidobacterium Species, Lactobacillus Species, andOther Lactic Acid Bacteria in the HumanColon

Published:2003-12 Issue:12 Volume:69 Page:7545-7548
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Nielsen D. S.¹,Møller P. L.¹,Rosenfeldt V.²,Pærregaard A.²,Michaelsen K. F.³,Jakobsen M.¹

Affiliation:

1. Department of Dairy and Food Science

2. University Clinic of Pediatrics, H:S Hvidovre Hospital, Hvidovre,Denmark

3. Department of Human Nutrition, Center for Advanced Food Studies, Royal Veterinary and Agricultural University, Frederiksberg

Abstract

ABSTRACT The distribution of mucosa-associated bacteria, bifidobacteria and lactobacilli and closely related lactic acid bacteria, in biopsy samples from the ascending, transverse, and descending parts of the colon from four individuals was investigated by denaturing gradient gel electrophoresis (DGGE). Bifidobacterial genus-specific, Lactobacillus group-specific, and universal bacterial primers were used in a nested PCR approach to amplify a fragment of the 16S rRNA gene. DGGE profiles of the bifidobacterial community were relatively simple, with one or two amplicons detected at most sampling sites in the colon. DGGE profiles obtained with Lactobacillus group-specific primers were complex and varied with host and sampling site in the colon. The overall bacterial community varied with host but not sampling site.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.69.12.7545-7548.2003

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