Disruption of the CAR1 Gene Encoding Arginase Enhances Freeze Tolerance of the Commercial Baker's Yeast Saccharomyces cerevisiae

Author:

Shima Jun1,Sakata-Tsuda Yuko1,Suzuki Yasuo2,Nakajima Ryouichi2,Watanabe Hajime2,Kawamoto Shinichi1,Takano Hiroyuki1

Affiliation:

1. National Food Research Institute, Tsukuba, Ibaraki 305-8642

2. Tokyo Laboratory of Fermentation and Food Technology, Oriental Yeast Co. Ltd., Itabashi, Tokyo 174-8505, Japan

Abstract

ABSTRACT The effect of intracellular charged amino acids on freeze tolerance in doughs was determined by constructing homozygous diploid arginase-deficient mutants of commercial baker's yeast. An arginase mutant accumulated higher levels of arginine and/or glutamate and showed increased leavening ability during the frozen-dough baking process, suggesting that disruption of the CAR1 gene enhances freeze tolerance.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

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