Affiliation:
1. Department of Plant Pathology and Microbiology
2. Department of Veterinary Anatomy and Public Health, Texas A&M University, College Station, Texas 77843
Abstract
ABSTRACT
We previously characterized
Tri1
, a gene required for hydroxylation of the C-8 position during trichothecene mycotoxin biosynthesis in
Fusarium sporotrichioides
NRRL 3299. Sequence analysis of the region surrounding
Tri1
revealed a gene, named
Tri16
, which could encode an acyltransferase. Unlike the wild-type parent strain NRRL 3299, which accumulates primarily T-2 toxin along with low levels of diacetoxyscirpenol (DAS) and neosolaniol (NEO) and trace amounts of 8-propionyl-neosolaniol (P-NEO) and 8-isobutyryl-neosolaniol (B-NEO), mutants containing a disruption of
Tri16
were blocked in the production of the three C-8 esterified compounds T-2 toxin, P-NEO, and B-NEO and accumulated the C-8-hydroxylated compound NEO along with secondary levels of DAS. These data indicate that
Tri16
encodes an acyltransferase that catalyzes the formation of ester side groups at C-8 during trichothecene biosynthesis. We also report the presence of a
Tri16
ortholog in
Gibberella pulicaris
R-6380 that is likely linked to a presumably inactive ortholog for
Tri1
.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Cited by
52 articles.
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