Identification and Functional Characterization of Sphingomonas macrogolitabida Strain TFA Genes Involved in the First Two Steps of the Tetralin Catabolic Pathway

Author:

Moreno-Ruiz Emilia1,Hernáez María José1,Martínez-Pérez Olga1,Santero Eduardo1

Affiliation:

1. Departamento de Genética, Facultad de Biología, Universidad de Sevilla, Seville, Spain

Abstract

ABSTRACT Five genes involved in the two initial steps of the tetralin biodegradation pathway of Sphingomonas macrogolitabida strain TFA have been characterized. ThnA1A2 and ThnA3A4, components of the ring-hydroxylating dioxygenase, were encoded in divergently transcribed operons. ThnA1, ThnA2, and ThnA3 were essential for tetralin ring-hydroxylating dioxygenase activity. ThnB was identified as a dehydrogenase required for tetralin biodegradation.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

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