Long-Term Stability of Human Genomic and Human Papillomavirus DNA Stored in BD SurePath and Hologic PreservCyt Liquid-Based Cytology Media-Reference-Cited by-同舟云学术

Long-Term Stability of Human Genomic and Human Papillomavirus DNA Stored in BD SurePath and Hologic PreservCyt Liquid-Based Cytology Media

Published:2013-08 Issue:8 Volume:51 Page:2702-2706
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Agreda Patricia M.¹,Beitman Gerard H.¹,Gutierrez Erin C.¹,Harris James M.¹,Koch Kristopher R.¹,LaViers William D.¹,Leitch Sharon V.¹,Maus Courtney E.¹,McMillian Ray A.¹,Nussbaumer William A.¹,Palmer Marcus L. R.¹,Porter Michael J.¹,Richart Gregory A.¹,Schwab Ryan J.¹,Vaughan Laurence M.¹

Affiliation:

1. BD Diagnostics, Sparks, Maryland, USA

Abstract

ABSTRACT We evaluated the effect of storage at 2 to 8°C on the stability of human genomic and human papillomavirus (HPV) DNA stored in BD SurePath and Hologic PreservCyt liquid-based cytology media. DNA retained the ability to be extracted and PCR amplified for more than 2.5 years in both medium types. Prior inability to detect DNA in archived specimens may have been due to failure of the extraction method to isolate DNA from fixed cells.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.00759-13

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