Impact of Laboratory Cross-Contamination on Molecular Epidemiology Studies of Tuberculosis

Author:

Martínez Miguel1,de Viedma Darío García2,Alonso María2,Andrés Sandra2,Bouza Emilio2,Cabezas Teresa3,Cabeza Isabel3,Reyes Armando1,Sánchez-Yebra Waldo1,Rodríguez Manuel4,Sánchez M. Isabel5,Rogado M. Cruz3,Fernández Rosa5,Peñafiel Teresa5,Martínez Juan6,Barroso Pilar7,Lucerna M. Ángeles3,Diez L. Felipe1,Gutiérrez Carmelo7

Affiliation:

1. Complejo Hospitalario Torrecárdenas, Almería, Spain

2. Hopital Gregorio Marañón, Madrid, Spain

3. Empresa Pública Hospital de Poniente, El Ejido, Almería, Spain

4. Hospital la Inmaculada, Huercal-Overa, Almería, Spain

5. Unidad de Tuberculosis de Poniente, Santa María del Águila, Almería, Spain

6. Centro Penitenciario el Acebuche, Almería, Spain

7. Distrito Atención Primaria Levante-Alto Almanzora, Huercal-Overa, Almería, Spain

Abstract

ABSTRACT Laboratory cross-contamination by Mycobacterium tuberculosis is known to be responsible for the misdiagnosis of tuberculosis, but its impact on other contexts has not been analyzed. We present the findings of a molecular epidemiology analysis in which the recent transmission events identified by a genotyping reference center were overestimated as a result of unnoticed laboratory cross-contamination in the original diagnostic laboratories.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference12 articles.

1. Carroll, N. M., M. Richardson, E. Engelke, M. de Kock, C. Lombard, and P. D. van Helden. 2002. Reduction of the rate of false-positive cultures of Mycobacterium tuberculosis in a laboratory with a high culture positivity rate. Clin. Chem. Lab. Med.40:888-892.

2. Criteria for Identification of Cross-Contamination of Cultures of Mycobacterium tuberculosis in Routine Microbiology Laboratories

3. Centers for Disease Control and Prevention 2000. Misdiagnoses of tuberculosis resulting from laboratory cross-contamination of Mycobacterium tuberculosis cultures—New Jersey, 1998. Morb. Mortal. Wkly. Rep.49:413-416.

4. False-Positive Mycobacterium tuberculosis Cultures in 44 Laboratories in The Netherlands (1993 to 2000): Incidence, Risk Factors, and Consequences

5. Extensive Cross-Contamination of Specimens with Mycobacterium tuberculosis in a Reference Laboratory

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