Twenty-Four-Hour Immunofluorescence Technique for the Detection of Salmonellae in Nonfat Dry Milk

Author:

Reamer R. H.1,Hargrove R. E.1

Affiliation:

1. Dairy Products Laboratory, Agricultural Research Service, Beltsville, Maryland 20705

Abstract

A detection procedure was developed in which a newly devised lysine-iron medium was used as a one-step selective and enrichment medium for detection of salmonellae by the fluorescent-antibody technique. Incubation was conducted in two steps: initially at 30 C for 5 hr to resuscitate sublethally stressed cells, followed by incubation at 39 C for 17 hr. Twenty-seven strains of salmonellae from groups A-I were utilized in the development of this procedure which was sensitive enough to detect one Salmonella bacterium in 100 g of nonfat dry milk.

Publisher

American Society for Microbiology

Subject

General Pharmacology, Toxicology and Pharmaceutics,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine

Reference13 articles.

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