Scanning electron microscopy of fruiting body formation by myxobacteria

Author:

Grilione P L,Pangborn J

Abstract

Scanning electron microscopy was used to follow fruiting body formation by pure cultures of Chondromyces crocatus M38 and Stigmatella aurantica. Vegetative cells were grown on SP agar and then transferred to Bonner salts agar for fructification. Fruiting in both species commences with the formation of aggregation centers which resemble a fried egg in appearance. In Chondromyces the elevated center or "yolk" region of the aggregation enlarges into a bulbous structure under which the stalk forms and lengthens. At maximum stalk height the bulb extends laterally as bud-like swellings appear. These are immature sporangia and are arranged in a distintive radial pattern around the top of the stalk. This symmetry is lost as more sporangia are formed. Stigmatella does not form a bulb; rather the yolk region of the aggregation center projects upward to form a column-like stalk which is nearly uniform in diameter throughout its length. At maximum stalk height, the terminus of the stalk develops an irregular pattern of bud-like swellings. These differentiate into sporangia. Stalks of 2-week-old mature fruiting bodies of both species appear to be cellular in composition. Stereomicrographs suggest orientation of these cells parallel to the long axis of the stalk. Stalks of 8-week-old fruiting bodies of Chondromyces were acellular and consisted of empty tubules, suggesting that the cells undergo degeneration with aging of the fruiting body.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Reference20 articles.

1. Evidence for the formation of cell aggregates by chemotaxis in the development of the cellular slime mold Dictyostelium discoideum;Bonner J. T.;J. Exp. Zool.,1947

2. Fruiting myxobacteria as viewed with a scanning electron microscope;Brockman E. R.;Int. J. Syst. Bacteriol.,1974

3. Regulation of development in Myxococcus xanthus: effect of 3':5'- cyclic AMP, ADP and nutrition;Campos J. M.;Proc. Natl. Acad. Sci. U.S.A.,1975

4. Dworkin M. 1973. Cell-cell interactions in the myxobacteria p. 125-142. In J. M. Ashworth and J. E. Smith (ed.) 23rd Symp. Soc. Gen. Microbiol. Cambridge University Press Cambridge.

5. Jahn E. 1924. Beitrage zur botanischen Protistologie. I. Die Polyangiden. Verlag von Gebriider Borntraeger Leipzig.

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3