Inhibition of the Subgenomic Hepatitis C Virus Replicon in Huh-7 Cells by 2′-Deoxy-2′-Fluorocytidine-Reference-Cited by-同舟云学术

Inhibition of the Subgenomic Hepatitis C Virus Replicon in Huh-7 Cells by 2′-Deoxy-2′-Fluorocytidine

Published:2004-02 Issue:2 Volume:48 Page:651-654
ISSN:0066-4804
Container-title:Antimicrobial Agents and Chemotherapy
language:en
Short-container-title:Antimicrob Agents Chemother

Author:

Stuyver Lieven J.¹,McBrayer Tamara R.¹,Whitaker Tony¹,Tharnish Phillip M.¹,Ramesh Mangala¹,Lostia Stefania¹,Cartee Leanne¹,Shi Junxing¹,Hobbs Ann¹,Schinazi Raymond F.²,Watanabe Kyoichi A.¹,Otto Michael J.¹

Affiliation:

1. Pharmasset, Inc., Tucker, Georgia 30084

2. Department of Pediatrics, Emory School of Medicine/Veterans Affairs Medical Center, Decatur, Georgia 30033

Abstract

ABSTRACT 2′-Deoxy-2′-fluorocytidine (FdC) is a potent inhibitor of the hepatitis C virus RNA replicon in culture, and FdC-5′-triphosphate is an effective inhibitor of the NS5B polymerase. Dynamic profiling of cell growth in an antiviral assay showed that FdC caused cytostasis due to an S-phase arrest. These observations demonstrate that FdC treatment is affecting both a viral target and a cellular target.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Pharmacology (medical),Pharmacology

Link

https://journals.asm.org/doi/pdf/10.1128/AAC.48.2.651-654.2004

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