SigF Controls Carotenoid Pigment Production and Affects Transformation Efficiency and Hydrogen Peroxide Sensitivity in Mycobacterium smegmatis-Reference-Cited by-同舟云学术

SigF Controls Carotenoid Pigment Production and Affects Transformation Efficiency and Hydrogen Peroxide Sensitivity in Mycobacterium smegmatis

Published:2008-12 Issue:23 Volume:190 Page:7859-7863
ISSN:0021-9193
Container-title:Journal of Bacteriology
language:en
Short-container-title:J Bacteriol

Author:

Provvedi Roberta¹,Kocíncová Dana²³,Donà Valentina⁴,Euphrasie Daniel³,Daffé Mamadou⁵,Etienne Gilles⁵,Manganelli Riccardo⁴,Reyrat Jean-Marc²³

Affiliation:

1. Dipartimento di Biologia, Università di Padova, Padua, Italy

2. Université Paris Descartes, Faculté de Médecine Paris Descartes

3. INSERM, U570, Paris Cedex 15, F-75730 France

4. Dipartimento di Istologia, Microbiologia e Biotecnologie Mediche, Università di Padova, Padua, Italy

5. Institut de Pharmacologie et de Biologie Structurale, Unité Mixte de Recherche du Centre National de la Recherche Scientifique et de l'Université Paul Sabatier (UMR5089), Departement Mécanismes Moléculaires des Infections Mycobactériennes, Toulouse cedex 04, France

Abstract

ABSTRACT Carotenoids are complex lipids that are known for acting against photodynamic injury and free radicals. We demonstrate here that σ F is required for carotenoid pigment production in Mycobacterium smegmatis . We further show that a sigF mutant exhibits a transformation efficiency 10 4 -fold higher than that of the parental strain, suggesting that σ F regulates the production of components affecting cell wall permeability. In addition, a sigF mutant showed an increased sensitivity to hydrogen peroxide. An in silico search of the M. smegmatis genome identified a number of SigF consensus sites, including sites upstream of the carotenoid synthesis locus, which explains its SigF regulation.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JB.00714-08

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