Affiliation:
1. NRC-Biotechnology Research Institute, Montreal, Quebec, Canada.
Abstract
Three hydrocarbon-degrading psychrotrophic bacteria were isolated from petroleum-contaminated Arctic soils and characterized. Two of the strains, identified as Pseudomonas spp., degraded C5 to C12 n-alkanes, toluene, and naphthalene at both 5 and 25 degrees C and possessed both the alk catabolic pathway for alkane biodegradation and the nah catabolic pathway for polynuclear aromatic hydrocarbon biodegradation. One of these strains contained both a plasmid slightly smaller than the P. oleovorans OCT plasmid, which hybridized to an alkB gene probe, and a NAH plasmid similar to NAH7, demonstrating that both catabolic pathways, located on separate plasmids, can naturally coexist in the same bacterium.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Reference28 articles.
1. Anderson J. P. E. 1982. Soil respiration p. 836-841. In A. L. Page (ed.) Methods of soil analysis part 2. Chemical and microbiological properties 2nd ed. American Society of Agronomy Madison Wis.
2. Rapid, direct extraction of DNA from soils for PCR analysis using polyvinylpolypyrrolidone spin columns;Berthelet M.;FEMS Microbiol. Lett.,1996
3. Naphthalene plasmids in pseudomonads;Conners M. A.;J. Bacteriol.,1982
4. Biodegradation by an Arthrobacter species of hydrocarbons partitioned into an organic solvent;Efroymson R. A.;Appl. Environ. Microbiol.,1991
5. Expression of naphthalene oxidation genes in Escherichia coli results in the biosynthesis of indigo;Ensley B. D.;Science,1983
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