Single-Step Multiplex PCR Assay for Determining 92 Pneumococcal Serotypes-Reference-Cited by-同舟云学术

Single-Step Multiplex PCR Assay for Determining 92 Pneumococcal Serotypes

Published:2016-08 Issue:8 Volume:54 Page:2197-2200
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Marimón José M.¹²,Ercibengoa María²,Santacatterina Erica³,Alonso Marta¹,Pérez-Trallero Emilio¹²⁴

Affiliation:

1. Microbiology Department, Hospital Universitario Donostia-Instituto de Investigación Sanitaria Biodonostia, San Sebastián, Spain

2. Biomedical Research Center Network for Respiratory Diseases (CIBERES), Madrid, Spain

3. Microbiology and Virology Unit, Padua University Hospital, Padua, Italy

4. Faculty of Medicine, University of the Basque Country, UPV/EHU, San Sebastián, Spain

Abstract

ABSTRACT For pneumococcal disease surveillance, simple and cost-effective methods capable of determining all serotypes are needed. Combining a single-tube multiplex PCR with fluorescently labeled primers followed by amplicon analysis using automated fluorescent capillary electrophoresis, each serotype of 92 reference isolates and 297 recently collected clinical isolates was successfully determined.

Funder

Health Department of the Basque Country Government

University of the Basque Country EHU/UPV

MINECO | Instituto de Salud Carlos III

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.01156-16

Reference23 articles.

1. Pneumococcal Capsules and Their Types: Past, Present, and Future