Quantitation of Integrated HIV Provirus by Pulsed-Field Gel Electrophoresis and Droplet Digital PCR-Reference-Cited by-同舟云学术

Quantitation of Integrated HIV Provirus by Pulsed-Field Gel Electrophoresis and Droplet Digital PCR

Published:2018-12 Issue:12 Volume:56 Page:
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Lada Steven M.¹²,Huang Karissa¹²,VanBelzen D. Jake³,Montaner Luis J.⁴,O'Doherty Una³,Richman Douglas D.¹⁵^ORCID

Affiliation:

1. San Diego Center for AIDS Research, University of California San Diego, La Jolla, California, USA

2. Veterans Medical Research Foundation, San Diego, California, USA

3. Department of Laboratory Medicine and Therapeutic Pathology, University of Pennsylvania, Philadelphia, Pennsylvania, USA

4. The Wistar Institute, Philadelphia, Pennsylvania, USA

5. Veterans Affairs San Diego Healthcare System, La Jolla, California, USA

Abstract

We utilized pulsed-field gel electrophoresis (PFGE) to purify high-molecular-weight DNA from HIV-infected cells. This purification, in combination with our previously described droplet digital PCR (ddPCR) assay, was used to develop a method to quantify proviral integrated HIV DNA free of lower-molecular-weight species of HIV DNA.

Funder

HHS | NIH | NIH Office of the Director

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.01158-18

Reference29 articles.

1. The Challenge of Finding a Cure for HIV Infection

2. Eradicating HIV-1 infection: seeking to clear a persistent pathogen

3. Latency reversal and viral clearance to cure HIV-1