Small, Acid-Soluble Proteins as Biomarkers in Mass Spectrometry Analysis of Bacillu s Spores-Reference-Cited by-同舟云学术

Small, Acid-Soluble Proteins as Biomarkers in Mass Spectrometry Analysis of Bacillu s Spores

Published:2003-02 Issue:2 Volume:69 Page:1100-1107
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Hathout Yetrib¹,Setlow Barbara²,Cabrera-Martinez Rosa-Martha²,Fenselau Catherine¹,Setlow Peter²

Affiliation:

1. Department of Chemistry and Biochemistry, University of Maryland, College Park, Maryland 20704

2. Department of Biochemistry, University of Connecticut Health Center, Farmington, Connecticut 06032

Abstract

ABSTRACT The use of 1 N HCl for extraction of small, acid-soluble proteins (SASP) from different Bacillus spore species was examined. The extracts were analyzed by high-performance liquid chromatography and matrix-assisted laser desorption mass spectrometry and were found to be both qualitatively and quantitatively superior to extraction by acetonitrile-5% trifluoroacetic acid (70:30, vol/vol). Both major and minor α/β- and γ-type SASP were characterized by their molecular masses or tryptic peptide maps and by searches of both protein and unannotated genome databases. For all but 1 pair ( B. cereus T and B. thuringiensis subsp. Kurstaki ) among the 11 variants studied the suites of SASP masses are distinctive, consistent with the use of these proteins as potential biomarkers for spore identification by mass spectrometry.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.69.2.1100-1107.2003

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