Role of p H and Nitrogen Limitation in the Elaboration of the Extracellular Polysaccharide Pullulan by Pullularia pullulans

Author:

Catley B. J.1

Affiliation:

1. Department of Biochemistry, University of Miami School of Medicine, Miami, Florida 33152

Abstract

During the growth of the yeastlike fungus Pullularia pullulans on glucose as sole carbon source, the cell-mass does not increase concomitantly with the elaboration of the extracellular polysaccharide pullulan, but precedes it. The conditions generated in the culture medium which activate the secretion of polysaccharide have been sought for, and, in particular, the roles of extracellular p H and nitrogen limitatation are examined.

Publisher

American Society for Microbiology

Subject

General Pharmacology, Toxicology and Pharmaceutics,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine

Reference11 articles.

1. The mechanism of carbohydrase action. II. Pullulanase, an enzyme specific for hydrolysis of a-1-6-bonds in amylaceous oligo- and polysaccharides;Abdullah M.;Cereal Chem.,1966

2. Pullulan, ein extracellulares Glucan von Pullularia pullulans;Bender H.;Biochim. Biophys. Acta,1959

3. Untersuchungen an Pullulan. II. Spezifischer Abbau durch ein bakterielles Enzym;Bender H.;Biochem. Z.,1961

4. Polysaccharides elaborated by Pullularia pullulanis. I. The neutral glucan synthesised from sucrose solutions;Bouveng H. O.;Acta Chem. Scan.,1962

5. Pullulan, a relationship between molecular weight and fine structure;Catley B. J.;FEBS Lett.,1970

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