Usefulness of Serial Blood Sampling and PCR Replicates for Treatment Monitoring of Patients with Chronic Chagas Disease-Reference-Cited by-同舟云学术

Usefulness of Serial Blood Sampling and PCR Replicates for Treatment Monitoring of Patients with Chronic Chagas Disease

Published:2019-02 Issue:2 Volume:63 Page:
ISSN:0066-4804
Container-title:Antimicrobial Agents and Chemotherapy
language:en
Short-container-title:Antimicrob Agents Chemother

Author:

Parrado Rudy¹,Ramirez Juan Carlos²,de la Barra Anabelle¹,Alonso-Vega Cristina³,Juiz Natalia²^ORCID,Ortiz Lourdes⁴,Illanes Daniel¹,Torrico Faustino⁵,Gascon Joaquim⁶,Alves Fabiana³,Flevaud Laurence⁷,Garcia Lineth¹,Schijman Alejandro G.²,Ribeiro Isabela³^ORCID

Affiliation:

1. Universidad Mayor de San Simón, Cochabamba, Bolivia

2. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular Dr. Héctor N. Torres (INGEBI-CONICET), Buenos Aires, Argentina

3. Drugs for Neglected Diseases initiative (DNDi), Geneva, Switzerland

4. Universidad Autónoma Juan Misael Saracho, Tarija, Bolivia

5. Fundación CEADES, Cochabamba, Bolivia

6. Barcelona Centre for International Health Research (CRESIB), Barcelona, Spain

7. Médicins Sans Frontières (MSF), Geneva, Switzerland

Abstract

This work evaluated a serial blood sampling procedure to enhance the sensitivity of duplex real-time quantitative PCR (qPCR) for baseline detection and quantification of parasitic loads and posttreatment identification of failure in the context of clinical trials for treatment of chronic Chagas disease, namely, DNDi-CH-E1224-001 (ClinicalTrials.gov registration no. NCT01489228) and the MSF-DNDi PCR Sampling Optimization Study (NCT01678599).

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Pharmacology (medical),Pharmacology

Link

https://journals.asm.org/doi/pdf/10.1128/AAC.01191-18

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