Affiliation:
1. Department of Food Science, Cornell University, Ithaca, New York 14853
Abstract
An
argB
mutant of
Aspergillus oryzae
NRRL 492 has been genetically transformed with the
Aspergillus nidulans argB
gene. Protoplasts were generated with a combination of Novozyme 234 and β-glucuronidase and regenerated on sucrose-stabilized minimal medium without arginine as described for
A. nidulans.
A frequency of 5 to 10 transformants per μg of DNA was obtained; however, most transformants appeared abortive. The
A. nidulans argB
gene and vector sequences appeared to be integrated into the
A. oryzae
chromosome.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Cited by
21 articles.
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