Influence of pH on microbial hydrogen metabolism in diverse sedimentary ecosystems

Abstract

Hydrogen transformation kinetic parameters were measured in sediments from anaerobic systems covering a wide range of environmental pH values to assess the influence of pH on hydrogen metabolism. The concentrations of dissolved hydrogen were measured and hydrogen transformation kinetics of the sediments were monitored in the laboratory by monitoring hydrogen consumption progress curves. The hydrogen turnover rate constants (kt) decreased directly as a function of decreasing sediment pH, and the maximum hydrogen uptake velocities (Vmax) varied as a function of pH within each of the trophic states. Conversely, the half-saturation concentrations (Km) were independent of pH. The steady-state hydrogen concentrations were at least 2 orders of magnitude lower than the half-saturation constants for hydrogen uptake. Dissolved hydrogen concentrations were at least fivefold higher in sediments from eutrophic systems than from oligotrophic and dystrophic systems. The rates of hydrogen production determined from the assumption of steady state decreased with sediment pH. These data indicate that progressively lower pH values inhibit microbial hydrogen-producing and -consuming processes within sedimentary ecosystems.