Affiliation:
1. Institut für Biotechnologie der Kernforschungsanlage Jülich, Postfach 1913, D-5170 Jülich 1, 2 and Institut für Technologie der Bundesforschungsanstalt für Landwirtschaft, D-3300 Braunschweig, Federal Republic of Germany
Abstract
The use of F
420
as a parameter for growth or metabolic activity of methanogenic bacteria was investigated. Two representative species of methanogens were grown in batch culture:
Methanobacterium bryantii
(strain M.o.H.G.) on H
2
and CO
2
, and
Methanosarcina barkeri
(strain Fusaro) on methanol or acetate. The total intracellular content of coenzyme F
420
was followed by high-resolution fluorescence spectroscopy. F
420
concentration in
M. bryantii
ranged from 1.84 to 3.65 μmol · g of protein
−1
; and in
M. barkeri
grown with methanol it ranged from 0.84 to 1.54 μmol · g
−1
depending on growth conditions. The content of F
420
in
M. barkeri
was influenced by a factor of 2 depending on the composition of the medium (minimal or complex) and by a factor of 3 to 4 depending on whether methanol or acetate was used as the carbon source. A comparison of F
420
content with protein, cell dry weight, optical density, and specific methane production rate showed that the intracellular content of F
420
approximately followed the increase in biomass in both strains. In contrast, no correlation was found between specific methane production rate and intracellular F
420
content. However, qCH
4
(F
420
), calculated by dividing the methane production rate by the coenzyme F
420
concentration, almost paralleled qCH
4
(protein). These results suggest that F
420
may be used as a specific parameter for estimating the biomass, but not the metabolic activity, of methanogens; hence qCH
4
(F
420
) determined in mixed populations with complex carbon substrates must be considered as measure of the actual methanogenic activity and not as a measure of potential activity.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Reference40 articles.
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