Affiliation:
1. 31 Bramble Walk, Lymington SO41 9LW, United Kingdom,1 and
2. Hygiene Institute, University of Vienna, A-1095 Vienna, Austria2
Abstract
SUMMARY
The contribution of enzyme tests to the accurate and rapid routine identification of gram-positive cocci is introduced. The current taxonomy of the genera of aerobic and facultatively anaerobic cocci based on genotypic and phenotypic characterization is reviewed. The clinical and economic importance of members of these taxa is briefly summarized. Tables summarizing test schemes and kits available for the identification of staphylococci, enterococci, and streptococci on the basis of general requirements, number of tests, number of taxa, test classes, and completion times are discussed. Enzyme tests included in each scheme are compared on the basis of their synthetic moiety. The current understanding of the activity of enzymes important for classification and identification of the major groups, methods of testing, and relevance to the ease and speed of identification are reviewed. Publications describing the use of different identification kits are listed, and overall identification successes and problems are discussed. The relationships between the results of conventional biochemical and rapid enzyme tests are described and considered. The use of synthetic substrates for the detection of glycosidases and peptidases is reviewed, and the advantages of fluorogenic synthetic moieties are discussed. The relevance of enzyme tests to accurate and meaningful rapid routine identification is discussed.
Publisher
American Society for Microbiology
Subject
Infectious Diseases,Microbiology (medical),Public Health, Environmental and Occupational Health,General Immunology and Microbiology,Epidemiology
Reference254 articles.
1. Achondo
K.
Bascomb
S.
Bobolis
J.
Chipman
A.
Connell
S.
Enscoe
G.
Gardner
B.
Mayhew
P.
Nothaft
D.
Skinner
J.
Stearn
L.
Williams
G.
Voong
J.
Abbott
S.
O’Hara
C.
Schreckenberger
P.
New improved MicroScan Rapid Negative Identification Panel abstr. C-307
Abstracts of the 95th General Meeting of the American Society for Microbiology 1995.
1995
53
American Society for Microbiology
Washington D.C
2. Phylogenetic analysis of some Aerococcus-like organisms from urinary tract infections: description of Aerococcus urinae sp. nov.;Aguirre M.;J. Gen. Microbiol.,1992
3. Phylogenetic analysis of Alloiococcus otitis gen. nov., sp. nov., an organism from human middle ear fluid.;Aguirre M.;Int. J. Syst. Bacteriol.,1992
4. Phenotypic and phylogenetic characterization of some Gemella-like organisms from human infections: description of Dolosigranulum pigrum gen. nov., sp. nov.;Aguirre M.;J. Appl. Bacteriol.,1993
5. Identification of coagulase-negative staphylococci with the API STAPH-IDENT system
Cited by
55 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献