Organization of the replication control region of plasmid ColIb-P9-Reference-Cited by-同舟云学术

Organization of the replication control region of plasmid ColIb-P9

Published:1990-04 Issue:4 Volume:172 Page:1983-1991
ISSN:0021-9193
Container-title:Journal of Bacteriology
language:en
Short-container-title:J Bacteriol

Author:

Hama C¹,Takizawa T¹,Moriwaki H¹,Urasaki Y¹,Mizobuchi K¹

Affiliation:

1. Department of Biophysics and Biochemistry, Faculty of Science, University of Tokyo, Japan.

Abstract

We identified a 1,845-base-pair sequence that contains essential information for the autonomous replication and regulation of the 93-kilobase-pair IncI alpha group ColIb-P9 plasmid. Biochemical and genetic analyses revealed that this sequence specifies at least two structural genes, designated repZ and inc. The repZ gene encodes a protein with a molecular weight of 39,000, which probably functions as an initiator for the ColIb-P9 replicon. The inc gene that phenotypically governs the incompatibility encodes an RNA with a size of about 70 bases. This small RNA acts in trans to repress the expression of repZ, thereby functioning to maintain a constant copy number of the ColIb-P9 replicon in host cells.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/jb.172.4.1983-1991.1990

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