Influence of Oxygen and pH on Methanethiol Production from l -Methionine by Brevibacterium linens CNRZ 918

Author:

Ferchichi Mohamed1,Hemme Denis1,Bouillanne Christian1

Affiliation:

1. Laboratoire de Microbiologie Laitière, Institut National de la Recherche Agronomique, Centre National de Recherches Zootechniques, 78350 Jouy-en-Josas, France

Abstract

The effects of dissolved oxygen concentration and pH on the growth of Brevibacterium linens CNRZ 918 and its production of methanethiol from l -methionine were investigated. Optimal specific methanethiol production was obtained at 25% saturation of dissolved oxygen and at a pH between 8 and 9, whereas optimal cell growth occurred at 50% oxygen saturation and when the pH was maintained constantly at 7. Methanethiol production by nonproliferating bacteria required the presence of l -methionine (7 mM) in the culture medium. This was probably due to the induction of enzyme systems involved in the process. The intracellular concentration of l -methionine seemed to play a key role in this process. B. linens CNRZ 918 tolerated alkaline pHs with a maximal growth pH of approximately 9. Its orange pigmentation seemed to depend on the presence of l -methionine in the culture medium and on the concentration of dissolved oxygen.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Reference35 articles.

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