Indirect Immunofluorescence Assay for Intra Vitam Diagnosis of Avian Bornavirus Infection in Psittacine Birds

Author:

Herzog Sibylle1,Enderlein Dirk23,Heffels-Redmann Ursula2,Piepenbring Anne2,Neumann Daniel2,Kaleta Erhard F.2,Müller Hermann3,Lierz Michael2,Herden Christiane4

Affiliation:

1. Institute of Virology, Justus-Liebig-University Giessen, Giessen, Germany

2. Clinic for Birds, Reptiles, Amphibians and Fish, Justus-Liebig-University Giessen, Giessen, Germany

3. Institute for Virology, Faculty of Veterinary Medicine, University of Leipzig, Leipzig, Germany

4. Institute of Veterinary Pathology, Justus-Liebig-University Giessen, Giessen, Germany

Abstract

ABSTRACT Different avian bornavirus (ABV) genotypes have recently been detected in psittacine birds with proventricular dilatation disease (PDD), an inflammatory fatal central and peripheral nervous system disorder. An indirect immunofluorescence assay (IIFA) for intra vitam demonstration of ABV-specific serum antibodies was established since reverse transcription-PCR (RT-PCR) assays may not detect all ABV variants.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference15 articles.

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2. Experimental induction of proventricular dilatation disease in cockatiels (Nymphicus hollandicus) inoculated with brain homogenates containing avian bornavirus 4

3. Herden, C., S. Herzog, T. Wehner, C. Zink, J. A. Richt, and K. Frese. 1999. Comparison of different methods of diagnosing Borna disease in horses post mortem, p. 286-290. In U. Wernery, J. Wade, J. A. Mumford, and O. R. Kaaden (ed.), Equine infectious diseases VIII. R&W Publications, Newmarket, United Kingdom.

4. Novel Borna Virus in Psittacine Birds with Proventricular Dilatation Disease

5. Kaaden, O. R., S. Lange, and B. Stiburek. 1982. Establishment and characterization of chicken embryo fibroblast clone LSCC-H32. In Vitro18:827-834.

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