Deciphering Cellodextrin and Glucose Uptake in <i>Clostridium thermocellum</i>-Reference-Cited by-同舟云学术

Deciphering Cellodextrin and Glucose Uptake in Clostridium thermocellum

Published:2022-10-26 Issue:5 Volume:13 Page:
ISSN:2150-7511
Container-title:mBio
language:en
Short-container-title:mBio

Author:

Yan Fei¹²³⁴⁵,Dong Sheng¹²³⁴⁵,Liu Ya-Jun¹²³⁴⁵,Yao Xingzhe¹²³⁴⁵,Chen Chao¹²³⁴⁵,Xiao Yan¹²³,Bayer Edward A.⁶⁷^ORCID,Shoham Yuval⁸,You Chun⁹,Cui Qiu¹²³⁴⁵,Feng Yingang¹²³⁴⁵^ORCID

Affiliation:

1. CAS Key Laboratory of Biofuels, Shandong Provincial Key Laboratory of Synthetic Biology, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, China

2. Shandong Energy Institute, Shandong, Qingdao, China

3. Qingdao New Energy Shandong Laboratory, Shandong, Qingdao, China

4. Dalian National Laboratory for Clean Energy, Shandong, Qingdao, China

5. University of Chinese Academy of Sciences, Beijing, China

6. Department of Biomolecular Sciences, The Weizmann Institute of Science, Rehovot, Israel

7. Department of Life Sciences and the National Institute for Biotechnology in the Negev, Ben-Gurion University of the Negev, Beer-Sheva, Israel

8. Department of Biotechnology and Food Engineering, Technion-Israel Institute of Technology, Haifa, Israel

9. Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, China

Abstract

Highly efficient sugar uptake is important to microbial cell factories, and sugar transporters are therefore of great interest in the study of industrially relevant microorganisms. Clostridium thermocellum is a lignocellulolytic bacterium known for its multienzyme complex, the cellulosome, which is of great potential value in lignocellulose biorefinery. In this study, we clarify the function and mechanism of substrate specificity of the five reported putative sugar transporters using genetic, biophysical, and structural methods.

Funder

National Natural Science Foundation of China

QIBEBT and Dalian National Laboratory For Clean Energy

Shandong Energy Insitute

QIBEBT Research Foundation

Strategic Priority Research Program of the Chinese Academy of Sciences

Qingdao Independent Innovation Major Project

Israel Science Foundation

Shandong Energy Institute, Single-Cell Center Project

Publisher

American Society for Microbiology

Subject