Across Functional Boundaries: Making Nonneutralizing Antibodies To Neutralize HIV-1 and Mediate Fc-Mediated Effector Killing of Infected Cells-Reference-Cited by-同舟云学术

Across Functional Boundaries: Making Nonneutralizing Antibodies To Neutralize HIV-1 and Mediate Fc-Mediated Effector Killing of Infected Cells

Published:2021-10-26 Issue:5 Volume:12 Page:
ISSN:2150-7511
Container-title:mBio
language:en
Short-container-title:mBio

Author:

Richard Jonathan¹²,Nguyen Dung N.³,Tolbert William D.³,Gasser Romain¹²,Ding Shilei¹,Vézina Dani¹²^ORCID,Yu Gong Shang¹⁴,Prévost Jérémie¹²,Gendron-Lepage Gabrielle¹,Medjahed Halima¹,Gottumukkala Suneetha³,Finzi Andrés¹²⁴^ORCID,Pazgier Marzena³^ORCID

Affiliation:

1. Centre de Recherche du CHUM, Montréal, Québec, Canada

2. Département de Microbiologie, Infectiologie et Immunologie, Université de Montréal, Montréal, Québec, Canada

3. Infectious Disease Division, Department of Medicine of Uniformed Services University of the Health Sciences, Bethesda, Maryland, USA

4. Department of Microbiology and Immunology, McGill University, Montreal, Quebec, Canada

Abstract

Highly conserved epitopes within the coreceptor binding site (CoRBS) and constant region 1 and 2 (C1-C2 or cluster A) are only available for antibody recognition after the HIV-1 Env trimer binds host cell CD4; therefore, they are not accessible on virions and infected cells, where the expression of CD4 is downregulated. Here, we have developed new antibody fusion molecules in which domains 1 and 2 of soluble human CD4 are linked with monoclonal antibodies of either the CoRBS or cluster A specificity.

Funder

HHS | National Institutes of Health

Publisher

American Society for Microbiology

Subject

Virology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/mBio.01405-21

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