Validation of the VirCapSeq-VERT system for differential diagnosis, detection, and surveillance of viral infections

Author:

Kapoor Vishal12ORCID,Briese Thomas13,Ranjan Amit1,Donovan William M.1,Mansukhani Mahesh M.4,Chowdhary Rashmi5,Lipkin W. Ian1346ORCID

Affiliation:

1. Center for Infection and Immunity, Mailman School of Public Health, Columbia University, New York, New York, USA

2. Rabindranath Tagore University, Bhopal, India

3. Department of Epidemiology, Mailman School of Public Health, Columbia University, New York, New York, USA

4. Department of Pathology and Cell Biology, Vagelos College of Physicians and Surgeons,Columbia University, New York, New York, USA

5. All India Institute for Medical Sciences (AIIMS), Bhopal, India

6. Department of Neurology, Vagelos College of Physicians and Surgeons, Columbia University, New York, New York, USA

Abstract

ABSTRACT There is a need in clinical microbiology and public health for assays that enable sensitive and rapid detection of infectious agents. Next Generation Sequencing (NGS) is increasingly used in the fields of oncology and personalized genome medicine but has not gained a wider acceptance for clinical microbiology due to operational and bioinformatics complexity, as well as lower sensitivity compared to agent-specific quantitative polymerase chain reaction (qPCR) assays. VirCapSeq-VERT is a positive selection system for detection, typing, and strain differentiation of both RNA and DNA viruses with sensitivity comparable to qPCR. Here we report the analytical and clinical validation of the VirCapSeq-VERT system for detection of viruses in plasma and nasal secretions that cause systemic and/or respiratory infections. IMPORTANCE Broad range assay for accurate and sensitive diagnostics.

Funder

Skoll Foundation

HHS | National Institutes of Health

U.S. Department of Health and Human Services

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

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