Affiliation:
1. Department of Biological Sciences, Purdue University, Lafayette, Indiana 47907
Abstract
Control of formation of the histidyl-transfer ribonucleic acid (tRNA) synthetase with an increased
K
m
for histidine was studied in a
hisS
mutant of
Salmonella typhimurium
. Histidine restriction of both the
hisS
and
hisS
+
strains resulted in a derepression of synthesis of histidyl-tRNA synthetase. When grown in a concentration less than the
K
m
(100 μg/ml) of
l
-histidine, the
hisS
mutant maintained a higher level of histidyl-tRNA synthetase than the
hisS
+
strain. Addition of excess amounts of
l
-histidine to the growth medium of the
hisS
mutant culture grown with 100 μg of
l
-histidine per ml resulted in a repression of histidyl-tRNA synthetase formation to equal that of the
hisS
+
strain grown in 100 μg of
l
-histidine per ml. These data confirm previous findings that histidine tRNA is involved in the repression of synthesis of histidyl-tRNA synthetase.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Cited by
19 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献