Mutants with Altered Glucose Repression of Amidase Enzymes in Aspergillus nidulans

Abstract

Aspergillus nidulans produces acetamidase and formamidase enzymes. The acetamidase is produced in reduced amounts during growth on glucose, whereas the formamidase is not greatly affected. Mutations in a gene, amdT , which affect glucose repression of amidases are described. One of these, amdT102 , causes the acetamidase to be no longer subject to glucose repression and also affects ability to synthesize formamidase. The other, amdT19 , results in both the formamidase and the acetamidase being subject to abnormally strong glucose repression, and also in increased maximal acetamidase activities. The dominance relationships at the amdT locus have been investigated. It is suggested that the amdT gene may play a positive role in controlling amidase synthesis.