Affiliation:
1. Institut für Molekulare Mikrobiologie und Biotechnologie, Westfälische Wilhelms-Universität Münster, 48149 Münster, Germany
Abstract
ABSTRACT
Different fermentation strategies were employed for the cultivation of a new poly(3-hydroxybutyrate)-accumulating thermophilic bacterium,
Chelatococcus
sp. strain MW10, with the aim of achieving high-cell-density (HCD) growth and high poly(3-hydroxybutyrate) [poly(3HB)] productivity. Enhanced cultivation was achieved by a cyclic fed-batch fermentation (CFBF) technique (42-liter scale). Maximal poly(3HB) productivity was obtained during the second cycle [16.8 ± 4.2 g poly(3HB)/liter]. At the end of CFBF (265 h), an HCD of up to 115.0 ± 4.3 g cell dry weight/liter was achieved.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Reference49 articles.
1. Ahn, W. S., S. J. Park, and S. Y. Lee. 2001. Production of poly(3-hydroxybutyrate) from whey by cell recycle fed-batch culture of recombinant Escherichia coli. Biotechnol. Lett.23:235-240.
2. Occurrence, metabolism, metabolic role, and industrial uses of bacterial polyhydroxyalkanoates
3. Braunegg, G., G. Lefebvre, and K. F. Genser. 1998. Polyhydroxyalkanoates, biopolyesters from renewable resources: physiological and engineering aspects. J. Biotechnol.65:127-161.
4. Braunegg, G., M. Koller, P. Varila, C. Kutschera, R. Bona, C. Hermann, P. Horvat, J. Neto, and L. Pereira. 2007. Production of plastics from waste derived from agrofood industry, p. 119-135. In M. Graziani and P. Fornasiero (ed.), Renewable resources and renewable energy: a global challenge. CRC Press, Taylor and Francis Group, Boca Raton, FL.
5. Bushell, M. E., M. Rowe, C. A. Avignone-Rossa, and J. N. Wardell. 2003. Cyclic fed-batch culture for production of human serum albumin in Pichia pastoris. Biotechnol. Bioeng.82:678-683.
Cited by
69 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献