Detection of the Novel Extended-Spectrum β-Lactamase OXA-161 from a Plasmid-Located Integron in Pseudomonas aeruginosa Clinical Isolates from Spain-Reference-Cited by-同舟云学术

Detection of the Novel Extended-Spectrum β-Lactamase OXA-161 from a Plasmid-Located Integron in Pseudomonas aeruginosa Clinical Isolates from Spain

Published:2009-12 Issue:12 Volume:53 Page:5288-5290
ISSN:0066-4804
Container-title:Antimicrobial Agents and Chemotherapy
language:en
Short-container-title:Antimicrob Agents Chemother

Author:

Juan C.¹²,Mulet X.¹,Zamorano L.¹,Albertí S.²,Pérez J. L.¹,Oliver A.¹²

Affiliation:

1. Servicio de Microbiología, Hospital Son Dureta

2. Área de Microbiología, Universitat de les Illes Balears, Institut Universitari de Investigació en Ciències de la Salut (IUNICS), Palma de Mallorca, Spain

Abstract

ABSTRACT Two clonally related P seudomonas aeruginosa isolates, recovered from two patients admitted to a pediatric intensive care unit, were found to harbor a new OXA-2 variant (Asn148Asp), designated OXA-161. The plasmid location of bla OXA-161 was demonstrated through electroporation to PAO1, and its codification in a class I integron (together with aacA4 ) was demonstrated through PCR and sequencing. bla OXA-2 and bla OXA-161 were cloned in parallel to demonstrate the extended-spectrum β-lactamase properties of OXA-161, conferring resistance to ceftazidime and reduced susceptibility to cefepime and aztreonam.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Pharmacology (medical),Pharmacology

Link

https://journals.asm.org/doi/pdf/10.1128/AAC.00822-09

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