Expression of Bacterial GshF in Pichia pastoris for Glutathione Production-Reference-Cited by-同舟云学术

Expression of Bacterial GshF in Pichia pastoris for Glutathione Production

Published:2012-08 Issue:15 Volume:78 Page:5435-5439
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Ge Shaolin¹²,Zhu Taicheng¹,Li Yin¹

Affiliation:

1. Institute of Microbiology, Chinese Academy of Sciences, Beijing, People's Republic of China

2. School of Life Sciences, University of Science and Technology of China, Hefei, People's Republic of China

Abstract

ABSTRACT Conventionally, two consecutive enzymatic reactions catalyzed by γ-glutamylcysteine synthetase and glutathione synthetase are most commonly used for glutathione production. Here we demonstrate that bacterial bifunctional GshF can be used for glutathione production in a eukaryotic system without accumulation of the intermediate γ-glutamylcysteine.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.00509-12

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