Affiliation:
1. D�partement de microbiologie, Facult� de m�decine, Universit� Laval, Qu�bec City, Canada.
Abstract
Aeromonas hydrophila isolated from food and drinking water was tested for pathogenicity by studying its hemolysis, hemagglutination, and cytotoxicity. Hemolysis, tested on erythrocytes from six different species, was more frequently seen with water isolates (64%) than with food isolates (48%). Hemagglutination was more frequently encountered with food isolates (92%) than with water isolates (73%). Cytotoxicity, evaluated on seven cell lines, was frequently observed with food isolates (92%) and with water isolates (73%). Heat treatment (56 degrees C for 10 min) of culture supernatant fluids inhibited the toxicity of some but not all toxin-producing isolates. Our results suggest that the human intestinal cell line HT-29 could be a useful complement for testing A. hydrophila exotoxins and for studying the enteropathogenicity of this species for humans.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Reference19 articles.
1. American Public Health Association. 1995. Standard methods for the examination of water and wastewater 19th ed. p. 953-964. American Public Health Association Washington D.C.
2. Hemagglutination properties and adherence ability of Aeromonas hydrophila;Atkinson H. M.;Infect. Immun.,1980
3. Interaction between aerolysin, erythrocytes, and erythrocyte membranes;Bernheimer A. W.;Infect. Immun.,1975
4. Hemagglutination patterns of Aeromonas spp. in relation to biotype and source;Burke V.;J. Clin. Microbiol.,1984
5. Correlation of enterotoxicity with biotype in Aeromonas spp;Burke V.;J. Clin. Microbiol.,1983
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