Optimization and Evaluation of the 30S-S11 rRNA Gene for Taxonomic Profiling of Oral Streptococci-Reference-Cited by-同舟云学术

Optimization and Evaluation of the 30S-S11 rRNA Gene for Taxonomic Profiling of Oral Streptococci

Published:2022-07-12 Issue:13 Volume:88 Page:
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

O’Connell Lauren M.¹,Blouin Thomas¹,Soule Ashlyn¹,Burne Robert A.²^ORCID,Nascimento Marcelle M.³,Richards Vincent P.¹^ORCID

Affiliation:

1. Department of Biological Sciences, College of Science, Clemson University, Clemson, South Carolina, USA

2. Department of Oral Biology, College of Dentistry, University of Florida, Gainesville, Florida, USA

3. Department of Restorative Dental Sciences, Division of Operative Dentistry, College of Dentistry, University of Florida, Gainesville, Florida, USA

Abstract

The microbiota associated with the initiation and progression of dental caries has yet to be fully characterized. Although much insight has been gained from 16S rRNA hypervariable region DNA sequencing, this approach has several limitations, including poor taxonomic resolution at the species level.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/aem.00453-22

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