Affiliation:
1. Department of Immunology, Nagoya University School of Medicine, Japan.
Abstract
Staphylococcal enterotoxin A (SEA)- or SEB-stimulated T-lymphocyte proliferation was suppressed by the addition of high numbers of murine peritoneal macrophages or rat peritoneal or alveolar macrophages, whereas lower numbers of murine peritoneal macrophages enhanced the T-lymphocyte response. Suppression was associated with the increase of accumulation of nitrite, a product of nitric oxide, in the culture supernatants. This macrophage-mediated suppression was totally reversed by the addition of NG-monomethyl-L-arginine, a homolog of L-arginine, indicating that macrophage-mediated suppression of T-lymphocyte proliferation was mediated through the nitric oxide-synthesizing pathway activity. Macrophages in large numbers spontaneously produced nitric oxide in culture supernatant fluids. By the addition of autologous or allogeneic spleen cells but not thymocytes to SEA- or SEB-stimulated macrophage culture, nitric oxide production was greatly increased. When T lymphocytes in spleen cells were killed by antibody before addition to macrophage culture, nitric oxide production was diminished to the basal level. These results suggest that in addition to the action to support the process of T-lymphocyte activation by SEA or SEB, macrophages display a feedback regulatory action on the SEA- or SEB-stimulated T-cell proliferative response by releasing nitric oxide through interaction between macrophages and activated T lymphocytes.
Publisher
American Society for Microbiology
Subject
Infectious Diseases,Immunology,Microbiology,Parasitology
Reference27 articles.
1. Nitric oxide production is required for murine resident peritoneal macrophages to suppress mitogen-stimulated T cell proliferation: role of IFN-y in the induction of the nitric oxide-synthesizing pathway;Albina J. E.;J. Immunol.,1991
2. Mechanisms by which activated macrophages inhibit Iymphocyte responses;Allison A. C.;Immunol. Rev.,1978
3. Cloned and expressed nitric oxide synthase structurally resembles cytochrome P-450 reductase;Bredt D. S.;Nature (London),1991
4. Isolation of nitric oxide synthetase, a calmodulin-requiring enzyme;Bredt D. S.;Proc. Natl. Acad. Sci. USA,1990
5. Release of nitrogen intermediates and reactive oxygen intermediates from mouse peritoneal macrophages;Ding A. H.;J. Immunol.,1988
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