The Madness of Microbiome: Attempting To Find Consensus “Best Practice” for 16S Microbiome Studies-Reference-Cited by-同舟云学术

The Madness of Microbiome: Attempting To Find Consensus “Best Practice” for 16S Microbiome Studies

Published:2018-04 Issue:7 Volume:84 Page:
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Pollock Jolinda¹²^ORCID,Glendinning Laura²^ORCID,Wisedchanwet Trong²,Watson Mick²^ORCID

Affiliation:

1. Animal and Veterinary Sciences, Scotland's Rural College (SRUC), Edinburgh, United Kingdom

2. The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Edinburgh, United Kingdom

Abstract

ABSTRACT The development and continuous improvement of high-throughput sequencing platforms have stimulated interest in the study of complex microbial communities. Currently, the most popular sequencing approach to study microbial community composition and dynamics is targeted 16S rRNA gene metabarcoding. To prepare samples for sequencing, there are a variety of processing steps, each with the potential to introduce bias at the data analysis stage. In this short review, key information from the literature pertaining to each processing step is described, and consequently, general recommendations for future 16S rRNA gene metabarcoding experiments are made.

Funder

RCUK | Biotechnology and Biological Sciences Research Council

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.02627-17

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