New method for detection of Candida albicans in human blood by polymerase chain reaction

Author:

Miyakawa Y1,Mabuchi T1,Fukazawa Y1

Affiliation:

1. Department of Microbiology, Yamanashi Medical College, Japan.

Abstract

We developed a new method for detection of Candida albicans cells in human blood by the polymerase chain reaction that amplifies a 125-bp region within a species-specific, multiple-copy DNA fragment. In preparing template DNA from C. albicans-containing blood, the addition of C. tropicalis cells, together with antibody which reacts with both cells, raised the sensitivity of detection of C. albicans to about 3 cells per 0.1 ml.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference27 articles.

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4. Fukazawa Y. 1989. Antigenic structure of Candida albicans: immunochemical basis of the serologic specificity of the mannans in yeasts p. 37-62. In G. Marquis (ed.) Immunology of fungal diseases. Marcel Dekker Inc. New York.

5. Kaufman L. and E. Reiss. 1986. Serodiagnosis of fungal diseases p. 446-466. In N. R. Rose H. Friedman and J. L. Fahey (ed.) Manual of clinical laboratory immunology 3rd ed. American Society for Microbiology Washington D.C.

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