Evaluation of a Novel Broad-Spectrum PCR-Multiplex Genotyping Assay for Identification of Cutaneous Wart-Associated Human Papillomavirus Types
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Published:2010-05
Issue:5
Volume:48
Page:1706-1711
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ISSN:0095-1137
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Container-title:Journal of Clinical Microbiology
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language:en
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Short-container-title:J Clin Microbiol
Author:
de Koning Maurits N. C.1, ter Schegget Jan1, Eekhof Just A. H.2, Kamp Marga1, Kleter Bernhard1, Gussekloo Jacobijn2, Feltkamp Mariet C. W.3, Bouwes Bavinck Jan Nico4, Purdie Karin J.5, Bunker Christopher B.6, Proby Charlotte M.7, Meys Rhonda6, Harwood Catherine A.5, Quint Wim G. V.1
Affiliation:
1. DDL Diagnostic Laboratory, Voorburg, Netherlands 2. Department of Public Health and Primary Care 3. Department of Medical Microbiology, Centre of Infectious Diseases, Leiden University Medical Centre, Leiden, Netherlands 4. Department of Dermatology, Leiden University Medical Centre, Leiden, Netherlands 5. Centre for Cutaneous Research, Institute of Cell and Molecular Science, Barts and the London School of Medicine and Dentistry, Queen Mary University of London, London, United Kingdom 6. Department of Dermatology, Chelsea and Westminster Hospital, Faculty and Division of Medicine, Imperial College, London, United Kingdom 7. Centre for Oncology and Molecular Medicine, University of Dundee, Ninewells Hospital and Medical School, Dundee, United Kingdom
Abstract
ABSTRACT
A large number of human papillomavirus (HPV) types, distributed over five papillomavirus genera, are detectable in the skin. HPV types belonging to the alpha, gamma, and mu genera have been detected in cutaneous warts. A state-of-the-art HPV genotyping assay for these cutaneous wart-associated HPV types does not exist although warts constitute a highly prevalent skin condition, especially in children (33%) and organ transplant recipients (45%). Cutaneous warts are again the focus of attention as their clinical relevance rises with the increasing number of chronically immunosuppressed patients. The objective of this study was to develop and evaluate a DNA-based genotyping system for all known cutaneous wart-related HPV types using PCR and Luminex xMAP technology. The broad-spectrum PCR amplified DNA of all known wart-associated HPV types from the genera alpha (HPVs 2, 3, 7, 10, 27, 28, 29, 40, 43, 57, 77, 91, and 94), gamma (HPVs 4, 65, 95, 48, 50, 60, and 88), mu (HPVs 1 and 63), and nu (HPV41). The probes were evaluated using plasmid HPV DNA and a panel of 45 previously characterized cutaneous wart biopsy specimens showing high specificity. HPV was also identified in 96% of 100 swabs from nongenital cutaneous warts. HPV types 1, 2, 27, and 57 were the most prevalent HPV types detected in 89% of the swabs. In conclusion, this Luminex-based genotyping system identifies all known cutaneous wart HPV types including phylogenetically related types, is highly HPV type specific, and is suitable for large-scale epidemiological studies.
Publisher
American Society for Microbiology
Subject
Microbiology (medical)
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