Morphological and Transcriptional Responses to CRISPRi Knockdown of Essential Genes in Escherichia coli-Reference-Cited by-同舟云学术

Morphological and Transcriptional Responses to CRISPRi Knockdown of Essential Genes in Escherichia coli

Published:2021-10-26 Issue:5 Volume:12 Page:
ISSN:2150-7511
Container-title:mBio
language:en
Short-container-title:mBio

Author:

Silvis Melanie R.¹,Rajendram Manohary²,Shi Handuo³^ORCID,Osadnik Hendrik¹,Gray Andrew N.¹,Cesar Spencer³,Peters Jason M.⁴^ORCID,Hearne Cameron C.¹,Kumar Parth¹,Todor Horia¹,Huang Kerwyn Casey²³⁵^ORCID,Gross Carol A.¹

Affiliation:

1. Department of Cell and Tissue Biology, University of California San Francisco, San Francisco, California, USA

2. Department of Bioengineering, Stanford University, Stanford, California, USA

3. Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, California, USA

4. Pharmaceutical Sciences Division, School of Pharmacy, University of Wisconsin—Madison, Madison, Wisconsin, USA

5. Chan Zuckerberg Biohub, San Francisco, California, USA

Abstract

Essential genes make up only ∼5 to 10% of the genetic complement in most organisms but occupy much of their protein synthesis and account for almost all antibiotic targets. Despite the importance of essential genes, their intractability has, until recently, hampered efforts to study them.

Funder

HHS | National Institutes of Health

National Science Foundation

Agilent Foundation

Stanford University

James S. McDonnell Foundation

Innovative Genomics Institute

Allen Discovery Center at Stanford on Systems Modeling of Infection

Chan Zuckerberg Initiative

Publisher

American Society for Microbiology

Subject