DNA replication in a diploid strain of Saccharomyces cerevisiae homozygous for the rad6-1 mutation

Author:

Haładus E,Swietlińska Z,Zaborowska D,Zuk J

Abstract

The generation time of a diploid strain homozygous for the rad6-1 mutation was 160 min, and the duration of the S phase was 80 min; in the parental heterozygote, these values were 90 and 40 min, respectively. Analysis of DNA sedimentation in an alkaline sucrose gradient revealed that heterozygote high-molecular-weight DNA appeared after 60 min, and homozygote high-molecular weight DNA only after a 100-min pulse.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

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