Production of Ligninases and Degradation of Lignin in Agitated Submerged Cultures of Phanerochaete chrysosporium

Author:

Jäger Alexander1,Croan Suki1,Kirk T. Kent1

Affiliation:

1. Forest Products Laboratory, Forest Service, U. S. Department of Agriculture, Madison, Wisconsin 53705

Abstract

Research on the extracellular hemeprotein ligninases of Phanerochaete chrysosporium has been hampered by the necessity to produce them in stationary culture. This investigation examined the effects of detergents on development of ligninase activity in agitated submerged cultures. Results show that addition of Tween 80, Tween 20, or 3-[(3-colamidopropyl)dimethylammonio]1-propanesulfonate to the cultures permits development of ligninase activity comparable to that routinely obtained in stationary cultures. The detergent-amended cultures express the entire ligninolytic system, assayed as the complete oxidation of [ 14 C]lignin to 14 CO 2 . The detergent effect is evidently not merely in facilitating release of extant enzyme. Development of ligninolytic activity in the agitated cultures, as in stationary cultures, is idiophasic. Ion-exchange fast protein-liquid chromatography indicated that the heme protein profiles in agitated and stationary cultures are very similar. These findings should make it possible to scale up production of ligninolytic enzymes in stirred tank fermentors.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Reference14 articles.

1. Production of cellulases and 3-glucosidase by shake culture of Scytalidium lignicola;Desai J. D.;J. Ferment. Technol.,1982

2. Factors involved in the regulation of a ligninase activity in Phanerochaete chrysosporium;Faison B. D.;Appl. Environ. Microbiol.,1985

3. Purification and characterization of an extracellular H202- requiring diarylpropane oxygenase from the white-rot basidiomycete, Phanerochaete chrysosporium;Gold M. H.;Arch. Biochem. Biophys.,1984

4. Preparation and microbial decomposition of synthetic 14C-lignins;Kirk T. K.;Proc. Natl. Acad. Sci. USA,1975

5. Influence of culture parameters on lignin metabolism by Phanerochaete chrysosporium;Kirk T. K.;Arch. Microbiol.,1978

Cited by 165 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3